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Plant Pigment Spectrophotometry: Measuring Pigment Absorbance in Flowers and Leaves

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In this lesson students explore the relationship between pigmentation and light absorbance. Students will use a spectrophotometer to analyze pigment samples and record the absorption spectrum for pigments found in leaves and flowers. Students work in groups to analyze samples and discuss the relationship between the colors of leaves and flowers, the pigments they contain, and the light they absorb or reflect. This lesson can be done as a stand alone lesson if pigments are available, but requires the separation of pigments beforehand and functions best as a follow up to the plant pigment chromatography lesson.

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Grades: 7th Grade, 8th Grade, 9th Grade, 10th Grade, 11th Grade, 12th Grade
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Students will combine science and art by using an alternative photographic process called cyanotypes or sun prints. After a short introduction to the history of cyanotypes and the process of creating