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Measuring the Absorbance of Chlorophyll Solutions at Different Concentrations

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In this lesson, students will extract chlorophyll from spinach leaves and make different concentrations following the dilution method. Students will learn to compare the absorbance values obtained at various wavelengths using a spectrophotometer. They will understand that absorbance of a substance is directly proportional to its concentration in a solution. The goal of the lesson is to include investigating the linearity of the relationship and gaining insights into how changes in chlorophyll concentration affect absorbance.

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In this lesson, students will collect flower and leaf samples from around their school campus and return to the lab to conduct chromatography to separate pigments in their samples. Students will learn

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Plants are the basis for nearly all agricultural production. Agricultural plant crops produce food, fiber, fuel, and aesthetically pleasing plants. Plants utilize energy from the Sun to convert water