Plant Pigment Chromatography: Do plants and Leaves Contain the Same Pigments?
by Scott Milne
In this lesson, students will collect flower and leaf samples from around their school campus and return to the lab to conduct chromatography to separate pigments in their samples. Students will learn about the electromagnetic spectrum, why leaves and flowers appear as the colors we see and what the functions of these colors are. Students will learn about a simple form of chromatography, paper chromatography, and analyze the pigment types found in each of their samples by calculating the Rf value of each pigment front. This lesson can lead nicely into a second lesson about plant pigment spectrophotometry and analysis of concentration of plant pigments, or can be taught as a stand alone lesson.