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Measuring the Absorbance of Chlorophyll Solutions at Different Concentrations

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In this lesson, students will extract chlorophyll from spinach leaves and make different concentrations following the dilution method. Students will learn to compare the absorbance values obtained at various wavelengths using a spectrophotometer. They will understand that absorbance of a substance is directly proportional to its concentration in a solution. The goal of the lesson is to include investigating the linearity of the relationship and gaining insights into how changes in chlorophyll concentration affect absorbance.

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Grades: 6th Grade, 7th Grade, 8th Grade
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This is an 8-lesson unit that is designed to be used together to learn about the health and diversity of your local watershed by placing leaf packs into a water source (natural or man-made ponds