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Microbial Serial Dilutions

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This lesson is part of a project to isolate bacteria from soil and test it for antibiotic activity. This can be the start of the project or you can do lesson 1 soil microbes first. Though you do have to do this step before proceeding with the other lessons. This project is a real research experience for students and can be modified so students ask their own questions (example: change the type of soil). This lesson is based on the Tiny Earth project. Duration is of three days and students work in groups. Teacher prep can be up to 4 hours in sterilizing equipment and materials for students.

Lesson Grade Level

10th Grade 11th Grade 12th Grade

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In this lesson, students will extract chlorophyll from spinach leaves and make different concentrations following the dilution method. Students will learn to compare the absorbance values obtained at