Measuring the Absorbance of Chlorophyll Solutions at Different Concentrations
by Reny Mathew
In this lesson, students will extract chlorophyll from spinach leaves and make different concentrations following the dilution method. Students will learn to compare the absorbance values obtained at various wavelengths using a spectrophotometer. They will understand that absorbance of a substance is directly proportional to its concentration in a solution. The goal of the lesson is to include investigating the linearity of the relationship and gaining insights into how changes in chlorophyll concentration affect absorbance.